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Not tested. Don't have the game anymore.

Feel free to post if it works / what trophies you get.

Since first posting, this has been confirmed as working, so some users have been so helpful as to make and post their saves at different parts of the game to help out other users.

Save at the end of the game on Hard difficulty - Credit to edge4eva32

Save at 239/240 Riddler Trophies - Credit to edge4eva32

Another platinum save, but for the Game of the Year edition - Credit to WhatsaN00B
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Based off of user's tests, you are able to get all of the trophies for the challenges, by completing one (1). Aswell, you can get the Perfect Knight and World's Greatest Detective by scanning the Warden's Office.

Megaupload and rapidshare links are broken.

How does @WhatsaN00B save work. He 100% the game leaving no room to pop the trophies.

For some reason i can resign the save file but when i get to the saved same select screen i can't choose the 100% save game because it is overlapped with the start new game and i have to start a new game instead of playing the 100% game save please help